Journal: Oncology Letters

Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

doi: 10.3892/ol.2018.8036

Figure Lengend Snippet: Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

Techniques: Control

Journal: Oncology Letters

Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

doi: 10.3892/ol.2018.8036

Figure Lengend Snippet: Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

Techniques: Staining, Control

Journal: Oncology Letters

Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

doi: 10.3892/ol.2018.8036

Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

Techniques: Expressing, Standard Deviation, Control

Journal: Oncology Letters

Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

doi: 10.3892/ol.2018.8036

Figure Lengend Snippet: Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

Techniques: Expressing, Derivative Assay, Inhibition, Control

Journal: Oncology Letters

Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

doi: 10.3892/ol.2018.8036

Figure Lengend Snippet: Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

Techniques: Expressing, Derivative Assay, Control

Journal: Oncology Letters

Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

doi: 10.3892/ol.2018.8036

Figure Lengend Snippet: Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

Techniques: Expressing, Derivative Assay, Control

Journal: Oncology Letters

Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

doi: 10.3892/ol.2018.8036

Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

Techniques: Expressing, Derivative Assay, Control, Standard Deviation